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By Martin J. Pfeiffer, Martin Stehling, Anna Jauch (auth.), Hossein Baharvand, Nasser Aghdami (eds.)

ISBN-10: 1617799394

ISBN-13: 9781617799396

ISBN-10: 1617799408

ISBN-13: 9781617799402

Advances in Stem mobilephone Research discusses fresh advances in stem phone technology, together with healing functions. This quantity covers such issues as biomanufacturing iPS cells for healing purposes, ideas for controlling stem mobilephone destiny judgements, in addition to present easy study in such components as germ line stem cells, genomics and proteomics in stem cellphone study. it's a necessary booklet for biology and medical scientists, specifically younger investigators and stem cellphone biology scholars who're newly getting into the realm of stem cells study. The editors desire that the recent wisdom and examine defined during this booklet can assist give a contribution to new treatments for a wide selection of ailments that almost immediately afflict humanity.

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Safe, high-throughput passaging protocols and improved identification and removal of cells with abnormal genomes, such as by high resolution single nucleotide polymorphism (SNP) or comparative genomic hybridization (CGH) analyses, are needed [75, 76]. Undifferentiated hPSCs also pose a safety concern in which they have the potential to form teratomas [77]. Residual undifferentiated cells can be removed from a mixed population of cells using immunodepletion with antibodies against pluripotency surface markers, including SSEA-5, CD9, CD30, CD50, CD90, and CD200 [78].

Cardiomyocytes were selected based on G418 resistance, and optimization of reactor operation yielded greater than 3 cardiomyocytes per ESC after 2 weeks of differentiation. Niebruegge et al. 6 9 109 cardiomyocytes from murine ESCs in a 2 l perfusion bioreactor, also using antibiotic selection [65]. These studies demonstrate the feasibility of scalable pluripotent stem cell differentiation processes, which will be improved upon implementation of new, defined differentiation protocols discussed in Sect.

Herszfeld D, Wolvetang E, Langton-Bunker E, Chung TL, Filipczyk AA, Houssami S, Jamshidi P, Koh K, Laslett AL, Michalska A, Nguyen L, Reubinoff BE, Tellis I, Auerbach JM, Ording CJ, Looijenga LH, Pera MF (2006) CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells. Nat Biotechnol 24(3):351–357 28. Thomson A, Wojtacha D, Hewitt Z, Priddle H, Sottile V, Di Domenico A, Fletcher J, Waterfall M, Corrales NL, Ansell R, McWhir J (2008) Human embryonic stem cells passaged using enzymatic methods retain a normal karyotype and express CD30.

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Advances in Stem Cell Research by Martin J. Pfeiffer, Martin Stehling, Anna Jauch (auth.), Hossein Baharvand, Nasser Aghdami (eds.)

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