By Philippa M. O'Brien
This finished selection of confirmed antibody phage demonstrate protocols good points authoritative tips that might let the nonspecialist effectively to hold them out. insurance spans the development of antibody libraries, the choice of antibody clones with the specified homes, and their amendment, expression, and purification. finished and hugely functional, Antibody Phage reveal: equipment and Protocols offers biochemists, molecular biologists, and immunologists with a gold-standard reference consultant to the winning isolation, amendment, and expression of recombinant antibodies utilizing latest strong phage exhibit know-how.
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Additional resources for Antibody Phage Display: Methods and Protocols (Methods in Molecular Biology Volume 178)
Biol. 265, 161–72. 171. Winter, G. (1989) Antibody engineering. Phil. Trans. R. Soc. Lond. B 324, 537–547. 172. Hoogenboom, H. , de Bruine, A. , Hufton, S. , Hoet, R. , Arends, J. , and Roovers, R. C. (1998) Antibody phage display technology and its applications. Immunotechnology 4, 1–20. Fab Library Construction Protocols 39 2 Standard Protocols for the Construction of Fab Libraries Michelle A. Clark 1. Introduction Fab libraries, in which light-chain (LC) and heavy-chain (HC) variableregion genes are cloned into a phagemid vector and subsequently displayed on the surface of the filamentous phage particle, have been widely used for the isolation of antibodies (Abs) with specificity for haptens, foreign antigens (Ags), and self Ags.
1993) Selection of an anti-IGF-1 Fab from a Fab phage library created by mutagenesis of multiple CDR loops. Gene 128, 103–109. 58. , and Borrebaeck, C. A. K. (1995) Domain libraries: synthetic diversity for de novo design of antibody V regions. Gene 160, 269–272. 59. , and Logtenberg, T. (1995) Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage antibody library. Proc. Natl. Acad. Sci. USA 92, 3938–3942. 60. Griffiths, A. , Williams, S. , Tomlinson, I.
107, 269–281. 39. Graus, Y. , de Baets, M. , Parren, P. , van Breda Vriesman, P. , and Burton, D. R. (1997) Human anti-nicotinic acetylcholine receptor recombinant Fab fragments isolated from thymus-derived phage display libraries from myasthenia gravis patients reflect predominant specificities in serum and block the action of pathogenic serum antibodies. J. Immunol. 158, 1919–1929. 40. Barbas, C. F. and Burton, D. R. (1996) Selection and evolution of high-affinity human antiviral antibodies. Trends Biotechnol.
Antibody Phage Display: Methods and Protocols (Methods in Molecular Biology Volume 178) by Philippa M. O'Brien