Get Handbook of Stem Cells, Two-Volume Set: Volume 1-Embryonic PDF

By Robert Lanza

ISBN-10: 0124366430

ISBN-13: 9780124366435

New discoveries within the box of stem phone study have often seemed within the information and in medical literature. study during this region supplies to guide to new treatments for melanoma, center illness, diabetes, and a wide selection of different ailments.

This two-volume reference integrates this fascinating sector of biology, combining the must haves for a normal knowing of grownup and embryonic stem cells, the instruments, equipment, and experimental protocols had to research and symbolize stem cells and progenitor populations, in addition to a presentation via the world's specialists of what's at present identified approximately each one particular organ approach.

The editors of the Handbook of Stem Cells contain: Robert Lanza, Helen Blau, John Gearhart, Brigid Hogan, Douglas Melton, Malcolm Moore, Roger Pedersen, E. Donnall Thomas, James Thomson, Catherine Verfaillie, Irving Weissman, and Michael West. The Editorial Board contains: W. French Anderson, Peter Andrews, Anthony Atala, Jose Cibelli, Giulio Cossu, Robert Edwards, Martin Evans, Elaine Fuchs, Margaret Fuller, Fred Gage, Richard Gardner, Margaret Goodell, Ronald eco-friendly, William Haseltine, Joseph Itskovitz-Eldor, Rudolf Jaenisch, Ihor Lemischka, Dame Anne McLaren, Richard Mulligan, Stuart Orkin, Martin Pera, Benjamin Reubinoff, Janet Rossant, Hans Scholer, Austin Smith, Evan Snyder, Davor Solter, Alan Trounson, and Leonard Zon.

This complete set might be a much-needed addition to the library of scholars and researchers alike.

* presents accomplished assurance in this hugely topical subject
* comprises contributions by way of the key professionals and most excellent names within the box of stem cellphone research
* The accompanying CD-ROM contains over 250 colour figures

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Read or Download Handbook of Stem Cells, Two-Volume Set: Volume 1-Embryonic Stem Cells; Volume 2-Adult & Fetal Stem Cells PDF

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Extra resources for Handbook of Stem Cells, Two-Volume Set: Volume 1-Embryonic Stem Cells; Volume 2-Adult & Fetal Stem Cells

Sample text

M. (1971). Human blastocysts grown in culture. Nature 229, 132–133. 48. H. (1978). The primate blastocyst and its environment. Uppsala J. Med. Sci. ), 39–50. 49. G. (1982). First stages of the development of the human ovum. In “Pfizer Colloquium, Maformations Congènitales des Mammifères,” (H. ). Massson et Cie, Paris. 50. J. (1984). Human chorionic gonadotrophin secreted by preimplantation embryos. Science 223, 816–818. 51. Uphoff, D. (1958). Preclusion of secondary phase of irradiation syndrome by inoculation of foetal haemopoietic tissue following lethal total body X irradiation.

Successive publications covering these topics were summarised in 2001 and 2002 by Edwards,2,3 providing the basis for this chapter. Coincidentally, preimplantation growth in mammals were increasing in number following initial work in 1890 by Heape,4 who obtained rabbit offspring after transferring marked blastocysts into recipient does. In the early 20th century, Lewis and Gregory5 and Lewis and Wright6 cultured rabbit and mouse embryos through one or several cleavages. Pincus and Enzmann7 released rabbit ovarian oocytes from their follicles into culture media and recorded their spontaneous maturation to metaphase 2 and the extrusion of the first polar body.

Gaining parental permission, we decided to use them to make human embryo stem cell lines in vitro. 25,49 It was now apposite to begin work on stem cells, since Bourn Hall had conceived more than 100 IVF children, and high-quality blastocysts were growing in vitro. 50 They cleaved well, but four soon arrested. The other two embryos revealed good trophoblast activity as assessed by HCGβ assays at 120 and 170 hours after insemination. Denuded of their zona pellucida, they expanded and attached to plastic culture dishes at 189 and 216 hours after insemination, respectively.

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Handbook of Stem Cells, Two-Volume Set: Volume 1-Embryonic Stem Cells; Volume 2-Adult & Fetal Stem Cells by Robert Lanza


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