Intrabodies: Basic Research and Clinical Gene Therapy - download pdf or read online

By Wayne A. Marasco (auth.), Wayne A. Marasco M.D., Ph.D. (eds.)

ISBN-10: 3662121190

ISBN-13: 9783662121191

ISBN-10: 3662121212

ISBN-13: 9783662121214

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Wu Y, Duan L, Zhu M et al. Binding of intracellular anti-Rev single chain variable fragments to different epitopes of human immunodeficiency virus type 1 rev: Variations in viral inhibition. J Virol1996; 70:3290-3297. 126. Glockshuber R, Malia M, Pfitzinger I et al. -Fragments. Biochem 1990; 29:1362-1367. 127. Rudikoff S, Pumphrey JG. Functional antibody lacking a variable-region disulfide bridge. Proc Natl Acad Sci USA 1986; 83:7875-7878. CHAPTER 2 Ph~e Libraries for Generation of Stngle Chain Fv Antibodies for Intracellular Immunization Marie Alix Poul and James D.

EMBO J 1990; 9:101-108. 122. Levy-Mintz P, Duan L, Zhang H et al. Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase. J Virol1996; 70:8821-8832. 123. Pfanner N, Meijer M. Mitochondrial biogenesis: The Tom and Tim machine. Curr Biol1997; 7:R10o-R103. 124. Lockhart DJ, Dong H, Byrne MC et al. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nature Biotech 1996; 14:1675-1680.

A. In phage vectors, three to five copies of the scFv fragment are displayed (one on each of the pill molecules). B. In phagemid vector, less than three copies are displayed because pili from the helper phage competes with the fusion protein for incorporation into the phage surface. Phagemid vectors can contain an amber codon between the antibody fragment gene and gene III. This makes it possible to easily switch between displayed and soluble antibody fragment simply by changing the host bacterial strain (see details in text).

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Intrabodies: Basic Research and Clinical Gene Therapy Applications by Wayne A. Marasco (auth.), Wayne A. Marasco M.D., Ph.D. (eds.)


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