By O. A. Koroleva, G. R. Roberts, M. L. Tomlinson, J. H. Doonan (auth.), Professor Dr. Toshiyuki Nagata, Dr. Ken Matsuoka, Professor Dr. Dirk Inzé (eds.)
It is our utmost excitement to offer a brand new e-book on tobacco BY-2 cells, Tobacco BY-2 Cells: From mobile Dynamics to Omics, because the 58th quantity within the booklet sequence Biotechnology in Agriculture and Forestry (BAF). It represents an ext- sion of the former booklet Tobacco BY-2 Cells,vol. 53ofthe BAF. furthermore, the content material is very assorted from the latter and comprises new issues, gleaned from the 1st overseas Symposium on Tobacco BY-2 Cells held on the Plant technological know-how heart of the RIKEN, Yokohama, geared up via Nagata, Matsuoka and Inze, in September 2004. To this symposium got here greater than 2 hundred humans from varied elements of the area to debate matters. even if many of the members to thepreviousvolumeof Tobacco BY-2 Cells gave talks on their matters, there have been many different audio system who provided new themes and ways. So we loved the symposium greatly. hence we made up our minds to bring together a brand new quantity on tobacco BY-2 cells along with those new themes. moreover, in the direction of the top of the symposium, our universal figuring out was once that the tobacco BY-2 mobile approach remains to be very important in plant biology, particularly for learning the dynamic good points of plant cells. we are hoping this quantity turns out to be useful for plant biologists. Contents of the ebook are as follows: in Chapters I. 1–I. 6, numerous facets of the phone cycle and mobile dynamics utilizing BY-2 cells are defined. In Ch- ters II. 1–II.
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Extra resources for Tobacco BY-2 Cells: From Cellular Dynamics to Omics
Geelen and D. Inzé A PPB matures when it reaches its most narrow width of approximately 2 µm, which is close to 10−20 min ahead of microtubule disassembly. GFP-tagged Medicago CDKA localizes in tobacco suspension cells to a very narrow band that may correspond to the matured PPB at the point just before breakdown (Weingartner et al. 2001). From earlier microinjection studies it had already been concluded that the CDK/cyclin B kinase complex causes disassembly of the PPB microtubules, while interphase cortical microtubules, spindle, and phragmoplast were left unaffected (Hush et al.
2003). Microtubule initiation has been observed using a GFP marker, microtubule end-binding protein 1 (EB1), that binds to both the plus and minus ends of microtubules (Chan et al. 2003, 2005). Despite the abscence of centrosomes in plants, some components such as γ -tubulin and the spindle pole body Spc98 from yeast (AtSpc98) are genetically conserved (Erhardt et al. 2002). The localization of γ -tubulin and AtSpc98 suggests that plant microtubules may be initiated not only at the cortex but also at the perinuclear area (Liu et al.
Likewise, the plasma membrane does not accumulate GFP-KCA1 and hence it was called the KCA1 depleted zone (KDZ). The KDZ persists throughout mitosis and depends on the formation of a PPB. During mitosis, the KDZ is independent from the actin and microtubule cytoskeleton. Therefore it may provide spatial information to the phragmoplast and perhaps controls cell plate positioning independent from the actin network. 3 The Phragmoplast The dividing plant cell produces a new separating cell wall between the daughter nuclei in a way that looks very different from that which can be microscopically observed in animal or yeast cells.
Tobacco BY-2 Cells: From Cellular Dynamics to Omics by O. A. Koroleva, G. R. Roberts, M. L. Tomlinson, J. H. Doonan (auth.), Professor Dr. Toshiyuki Nagata, Dr. Ken Matsuoka, Professor Dr. Dirk Inzé (eds.)